Coding

Part:BBa_K3560001:Design

Designed by: ShiDi Xiao   Group: iGEM20_XHD-Wuhan-China   (2020-10-21)


gabY


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 138
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 52
    Illegal NgoMIV site found at 144
    Illegal NgoMIV site found at 310
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

gabY is derived from a pathogenic bacteria, so we cannot clone gabY from the strain. We found the gabY sequence on NCBI, and synthesized the gabY gene fragment by providing the sequence to the biosynthesis company.


Source

We know the sequence of gabY gene, synthesize the gabY gene fragment through a biological company.

References